*Applications may be reviewed on a rolling-basis and this posting could close before the deadline. Click here for information about the selection process.
EPA Office/Lab and Location: A research opportunity is available at the Environmental Protection Agency (EPA), Office of Research and Development (ORD), Center for Computational Toxicology and Exposure (CCTE), Great Lakes Toxicology & Ecology Division (GLTED) located in Duluth, Minnesota.
Research Project: The US EPA is mandated to address the endocrine disrupting potential of thousands of chemicals. For the majority of these chemicals there is a lack of information regarding whether they have the potential to cause adverse effects from impacts on thyroid hormone status. Understanding the important targets within the network of processes involved in maintaining normal functioning thyroid hormone levels, and what approaches to best assess activity at those targets are critical to developing a comprehensive approach to evaluating thyroid hormone disrupting potential of large numbers of chemicals.
Recently, a number of thyroid-related in vitro high-throughput screening (HTS) assays have been developed to broaden assay coverage for molecular targets potentially leading to thyroid disruption and were implemented to screen US EPA’s ToxCast Phase 1, 2 and E1K libraries. This battery of thyroid-related in vitro HTS assays can be employed to rapidly assess potential chemical toxicity toward these targets. However, this screening approach leaves uncertainties regarding the meaning of in vitro activity data as it relates to in vivo outcomes. Part of this research effort aims to develop a translational process that integrates relevant information, models, and data streams to provide richer context to in vitro results related to thyroid disruption. To that end, targeted in vivo testing will be performed using a model amphibian species (Xenopus sp.) routinely used for testing thyroid disrupting chemicals, to understand whether, and how, in vitro activity of chemicals translates to in vivo outcomes. Additionally, this research will employ existing computational tools such as the octanol-water partition coefficient (logKow) and quantitative structure-activity relationships (QSAR) models to provide deeper interpretation of in vitro data and further prioritize chemicals most likely to impact thyroid function in aquatic systems. The translational principles demonstrated will have much broader applicability across ToxCast HTS assays and aquatic vertebrates. This research will provide translational context necessary for ecological risk assessment and deliver timely information relative to PFAS thyroid toxicity. Coordinated efforts will be undertaken in the mammalian model in parallel to these efforts to bolster cross-species concordance of modes of action and adverse outcome pathways (AOPs) related to thyroid disruption.
Learning Objectives: The participant will be integrated into a transdisciplinary research team and engaged in multiple aspects of project planning, communication and coordination, research implementation, and analysis. The participant will have the opportunity to interact with internationally recognized leaders, both within and outside EPA. The participant may contribute to and/or publish original research. The participant will learn a diversity of laboratory, analytical, and computational techniques which can be applied across life sciences research fields. The research participant will learn to operate and use a range of scientific equipment. The participants will learn to evaluate data quality, trouble shoot research results, apply statistical methods for data analysis and interpretation.
Mentor(s): The mentor for this opportunity is Sigmund Degitz (firstname.lastname@example.org). If you have questions about the nature of the research please contact the mentor(s).
Anticipated Appointment Start Date: Spring 2021. All start dates are flexible and vary depending on numerous factors. Click here for detailed information about start dates.
Appointment Length: The appointment will initially be for one year and may be renewed up to four additional years upon EPA recommendation and subject to availability of funding.
Level of Participation: The appointment is full-time.
Participant Stipend: The participant will receive a monthly stipend commensurate with educational level and experience. Click here for detailed information about full-time stipends.
EPA Security Clearance: Completion of a successful background investigation by the Office of Personnel Management (OPM) is required for an applicant to be on-boarded at EPA.
ORISE Information: This program, administered by ORAU through its contract with the U.S. Department of Energy (DOE) to manage the Oak Ridge Institute for Science and Education (ORISE), was established through an interagency agreement between DOE and EPA. Participants do not become employees of EPA, DOE or the program administrator, and there are no employment-related benefits. Proof of health insurance is required for participation in this program. Health insurance can be obtained through ORISE.
Questions: Please see the FAQ section of our website. After reading, if you have additional questions about the application process please email ORISE.EPA.ORD@orau.org and include the reference code for this opportunity.
The qualified candidate should have received a doctoral degree in one of the relevant fields, or be currently pursuing the degree and will reach completion by the start of the appointment. Degree must have been received within five years of the appointment start date.