Zika Molecular Detection Fellowship - CDC

Organization
Centers for Disease Control and Prevention (CDC)
Reference Code
CDC-NCEZID-2017-0120
How to Apply

A complete application consists of:

All documents must be in English or include an official English translation.

If you have questions, send an email to CDCrpp@orau.org. Please include the reference code for this opportunity in your email.

Description

A research opportunity is available at the Centers for Disease Control and Prevention (CDC), National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), in Atlanta, Georgia.

The Division of High-Consequence Pathogens and Pathology (DHCPP), maximizes public health and safety nationally and internationally through the diagnosis, prevention, and control of disease, disability, and death caused by suspected and known viral, bacterial, prion, and related infections. DHCPP carries out the mission of NCEZID by providing leadership, expertise, and service in laboratory and epidemiological science, bioterrorism preparedness, applied research, diseases surveillance, outbreak response, policy development, health communication, education and training and program implementation and evaluation. DHCPP supports all these activities within the National Center as they apply to viral, bacterial, prion and related infections.

The Infectious Diseases Pathology Branch (IDPB), is the primary CDC program to provide tissue-based diagnosis of infectious diseases. Since the beginning of Zika virus (ZIKV) emergence in the Americas, IDPB has been deeply involved in the response efforts and unremittingly receiving formalin-fixed, paraffin-embedded (FFPE) tissue specimens for ZIKV testing. Because of shorter duration of ZIKV viremia and generally longer persist of ZIKV RNA in tissues, molecular assays help to expand diagnostic opportunities for congenitally-acquired and pregnancy-associated ZIKV infections and particularly valuable when conventional specimens are unavailable.

The fellow will gain experience by training in specialized molecular techniques such as RNA extraction from FFPE tissues using manual and automated tissue extractions, fresh tissue extractions, in-situ hybridization (ISH), real-time and conventional RT-PCR assays  for the detection of Zika Virus in tissues and latest DNA sequencing technologies

Specific Tasks may include:

  1. Conducting RNA extractions from FFPE tissues from suspect Zika virus cases.
  2. Performing quality control conventional and real-time RT-PCRs.
  3. Performing Zika virus conventional and real-time polymerase chain reaction (RT-PCR) assays.
  4. Performing gel purification and nucleotide sequencing of PCR.
  5. Performing sectioning of paraffin-embedded tissue blocks for PCR and ISH, as needed.
  6. Performing Zika virus ISH  assays, as needed.
  7. Recording and maintaining hardcopy and electronic copies of laboratory notebooks detailing experiments and results.
  8. Preparing inventories for Zika virus RNA extracts, helps in the ordering/maintenance of supplies.
  9. Performing reviews of literature related to the Zika virus and assist in data compilation and preparation of manuscripts/presentations.

All the above mentioned tasks will be performed under the guidance of the Molecular Pathology team lead.

The fellow will have opportunities to attend IDPB case review sessions, CDC seminars, training courses and DHCPP weekly seminars to enhance existing knowledge in microbiology/biological sciences, epidemiology and public health.

This program, administered by ORAU through its contract with the U.S. Department of Energy to manage the Oak Ridge Institute for Science and Education, was established through an interagency agreement between DOE and CDC. The initial appointment is for one year, but may be renewed upon recommendation of CDC contingent on the availability of funds. The participant will receive a monthly stipend commensurate with educational level and experience. Proof of health insurance is required for participation in this program. The appointment is full-time at CDC in the Atlanta, GA area. Participants do not become employees of CDC, DOE or the program administrator, and there are no employment-related benefits.

Qualifications

Applicants must have completed the requirements for a master’s or PhD degree in microbiology, biology, or related field that included at least 20 semester hours in microbiology or other subjects related to the study of microorganisms within five years of the start date of the appointment, or expect to complete all requirements for such a degree prior to starting the fellowship.  

Experience and proficiency in using various molecular biology techniques, including DNA and RNA extractions, conventional and real-time PCR/RT-PCR, gel electrophoresis, gel purification and DNA sequencing is required. Additional knowledge/experience of specialized techniques/tools, including in-situ hybridization, sectioning of paraffin-embedded tissue blocks, pyrosequencing, knowledge of sequence and data analysis software, scientific database search tools and reference management programs is also desirable.

Eligibility Requirements
  • Degree: Master's Degree or Doctoral Degree received within the last 60 month(s).
  • Discipline(s):
    • Life Health and Medical Sciences (4 )
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